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construct 962 human cyclin d1 promoter pgl3basic  (Addgene inc)


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    Structured Review

    Addgene inc construct 962 human cyclin d1 promoter pgl3basic
    List of selective cell cycle regulatory genes with symbol, functions, and folds change in high and low ETV4-expressing PC cells
    Construct 962 Human Cyclin D1 Promoter Pgl3basic, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/construct 962 human cyclin d1 promoter pgl3basic/product/Addgene inc
    Average 94 stars, based on 1 article reviews
    construct 962 human cyclin d1 promoter pgl3basic - by Bioz Stars, 2026-04
    94/100 stars

    Images

    1) Product Images from "ETV4 Facilitates Cell Cycle Progression in Pancreatic Cells through Transcriptional Regulation of Cyclin D1"

    Article Title: ETV4 Facilitates Cell Cycle Progression in Pancreatic Cells through Transcriptional Regulation of Cyclin D1

    Journal: Molecular cancer research : MCR

    doi: 10.1158/1541-7786.MCR-17-0219

    List of selective cell cycle regulatory genes with symbol, functions, and folds change in high and low ETV4-expressing PC cells
    Figure Legend Snippet: List of selective cell cycle regulatory genes with symbol, functions, and folds change in high and low ETV4-expressing PC cells

    Techniques Used:

    (A) Cyclin D1 cDNA construct (pCMV6-CCND1) was transfected in ETV4-silenced Colo357 and ASPC1 cells for its overexpression and confirmed by immunoblot analyses. (B) After 48 h of transfection, cells further transfected with Cyclin D1 promoter reporter construct and transcriptional activity of Cyclin D1 was examined. Data are presented as normalized relative luciferase activity (mean ± SD; n = 3, *p < 0.05). (C-D) Overexpression of cyclin D1 in ETV4-silenced cells rescued the inhibitory effect of ETV4-silencing on (C) growth and (D) cell cycle progression of PC cells.
    Figure Legend Snippet: (A) Cyclin D1 cDNA construct (pCMV6-CCND1) was transfected in ETV4-silenced Colo357 and ASPC1 cells for its overexpression and confirmed by immunoblot analyses. (B) After 48 h of transfection, cells further transfected with Cyclin D1 promoter reporter construct and transcriptional activity of Cyclin D1 was examined. Data are presented as normalized relative luciferase activity (mean ± SD; n = 3, *p < 0.05). (C-D) Overexpression of cyclin D1 in ETV4-silenced cells rescued the inhibitory effect of ETV4-silencing on (C) growth and (D) cell cycle progression of PC cells.

    Techniques Used: Construct, Transfection, Over Expression, Western Blot, Activity Assay, Luciferase



    Similar Products

    construct 962 human cyclin d1 promoter pgl3basic  (Addgene inc)
    94
    Addgene inc construct 962 human cyclin d1 promoter pgl3basic
    List of selective cell cycle regulatory genes with symbol, functions, and folds change in high and low ETV4-expressing PC cells
    Construct 962 Human Cyclin D1 Promoter Pgl3basic, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/construct 962 human cyclin d1 promoter pgl3basic/product/Addgene inc
    Average 94 stars, based on 1 article reviews
    construct 962 human cyclin d1 promoter pgl3basic - by Bioz Stars, 2026-04
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    List of selective cell cycle regulatory genes with symbol, functions, and folds change in high and low ETV4-expressing PC cells

    Journal: Molecular cancer research : MCR

    Article Title: ETV4 Facilitates Cell Cycle Progression in Pancreatic Cells through Transcriptional Regulation of Cyclin D1

    doi: 10.1158/1541-7786.MCR-17-0219

    Figure Lengend Snippet: List of selective cell cycle regulatory genes with symbol, functions, and folds change in high and low ETV4-expressing PC cells

    Article Snippet: The construct -962 human cyclin D1 promoter pGL3Basic (Addgene plasmid #32727) was from Frank McCormick laboratory and was procured through Addgene.

    Techniques:

    (A) Cyclin D1 cDNA construct (pCMV6-CCND1) was transfected in ETV4-silenced Colo357 and ASPC1 cells for its overexpression and confirmed by immunoblot analyses. (B) After 48 h of transfection, cells further transfected with Cyclin D1 promoter reporter construct and transcriptional activity of Cyclin D1 was examined. Data are presented as normalized relative luciferase activity (mean ± SD; n = 3, *p < 0.05). (C-D) Overexpression of cyclin D1 in ETV4-silenced cells rescued the inhibitory effect of ETV4-silencing on (C) growth and (D) cell cycle progression of PC cells.

    Journal: Molecular cancer research : MCR

    Article Title: ETV4 Facilitates Cell Cycle Progression in Pancreatic Cells through Transcriptional Regulation of Cyclin D1

    doi: 10.1158/1541-7786.MCR-17-0219

    Figure Lengend Snippet: (A) Cyclin D1 cDNA construct (pCMV6-CCND1) was transfected in ETV4-silenced Colo357 and ASPC1 cells for its overexpression and confirmed by immunoblot analyses. (B) After 48 h of transfection, cells further transfected with Cyclin D1 promoter reporter construct and transcriptional activity of Cyclin D1 was examined. Data are presented as normalized relative luciferase activity (mean ± SD; n = 3, *p < 0.05). (C-D) Overexpression of cyclin D1 in ETV4-silenced cells rescued the inhibitory effect of ETV4-silencing on (C) growth and (D) cell cycle progression of PC cells.

    Article Snippet: The construct -962 human cyclin D1 promoter pGL3Basic (Addgene plasmid #32727) was from Frank McCormick laboratory and was procured through Addgene.

    Techniques: Construct, Transfection, Over Expression, Western Blot, Activity Assay, Luciferase